390. Phage c31 Integrase Induces Chromosomal Aberrations in Primary Human Fibroblasts

Induction of chromosomal aberrations in human primary fibroblasts and immortalized cancer cells exposed to extremely-low-frequency electromagnetic fields

Background: Rapidly increasing possibilities of exposure to environmental extremely low-frequency electromagnetic fields (ELF-EMF) have become a topic of worldwide investigation. Epidemiological and laboratory studies suggest that exposure to ELF-EMF may increase cancer risk therefore assessment of chromosomal damage in various cell lines might be of predictive value for future risk es...

PhiC31 integrase induces a DNA damage response and chromosomal rearrangements in human adult fibroblasts

BACKGROUND PhiC31 integrase facilitates efficient integration of transgenes into human and mouse genomes and is considered for clinical gene therapy. However recent studies have shown that the enzyme can induce various chromosomal abnormalities in primary human embryonic cells and mammalian cell lines. The mechanisms involved are unknown, but it has been proposed that PhiC31 attachment sites in...

induction of chromosomal aberrations in human primary fibroblasts and immortalized cancer cells exposed to extremely-low-frequency electromagnetic fields

background: rapidly increasing possibilities of exposure to environmental extremely low-frequency electromagnetic fields (elf-emf) have become a topic of worldwide investigation. epidemiological and laboratory studies suggest that exposure to elf-emf may increase cancer risk therefore assessment of chromosomal damage in various cell lines might be of predictive value for future risk estimation....

Human Genetics – Chromosomal Aberrations

Autophagy Impairment Induces Premature Senescence in Primary Human Fibroblasts

BACKGROUND Recent studies have demonstrated that activation of autophagy increases the lifespan of organisms from yeast to flies. In contrast to the lifespan extension effect in lower organisms, it has been reported that overexpression of unc-51-like kinase 3 (ULK3), the mammalian homolog of autophagy-specific gene 1 (ATG1), induces premature senescence in human fibroblasts. Therefore, we asses...